Microscopy staining 2

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MICROSCOPY & STAINING1MICROSCOPY• The field of microbiology emerged from thedevelopment of the microscope, and the newfound ability to see microorganisms.• Fundamentally, visualisation of microorganismsrequires a microscope – this is the most basic toolin microbiology.• There are several types of microscopy – theyinclude light, electron, and scanned-probemicroscopy.2• Light microscopy views cells at a lowmagnification• Electron and scanned-probe are able to viewcells and cell structures at a very highmagnification.31. Compound light microscopy uses visible light toilluminate specimens. Types used inmicrobiology include:Brightfield, phase-contrast, differential interferencecontrast (DIC), dark-field, fluorescence, and confocal.2. Electron microscopy uses electrons instead ofvisible light for illumination. Types are:Transmission electron microscopy (TEM) and scanningelectron microscopy (SEM).3. Scanned-probe microscopy uses probes toexamine surfaces of specimens. Example:Atomic force microscopy (AFM) and Scanning TunnellingMicroscopy (STM):4LIGHT MICROSCOPYA TYPICALCOMPOUNDLIGHTMICROSCOPEComposed of:- Two lenses; objective & ocular (= compound)- A light source and condenser5Some key terms-Magnification: Ratio of an specimen’s image size to its realsize.Total magnification: The product of magnification of ocularand objective lenses.Resolution: The minimum distance that distinguishes (or theabilit … Purchase document to see full attachment

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