Methods of protein purification

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TOPIC – METHODS OF PROTEINPURIFICATIONBIOCHEMISTRY PRESENTATIONWHAT IS PROTEIN PURIFICATIONProtein purification is a series of processesor stepsapplied or intended to isolate one or a fewproteins from a complex mixture; usually cells, tisues or whole organism.IMPORTANCE OF PROTEINPURIFICATION IN BIOCHEMISTRYProtein purification is vital for theCharacterization of the function,structureand interactions of the proteins of interest.AIM FOR PROTEIN PURIFICATIONProtein purification involves isolating proteins from thesource based on differences in their physicalproperties.The objective of the protein purification scheme is toretain the largest amount of the functional protein withfewest contaminants.WHAT ARE PURIFIED PROTEINS USEDFOR.Purified proteins serve as extremely valuablebiochemical reagents.It is remarkably valuable to be able to obtain things likepurified growth factors or hormones, proteases, DNApolymerases, reverse transcriptase, ligases,phosphatases or antibodies that recognize a particularepitope of interest.METHODS OF PROTEIN PURIFICATIONThe four methods of protein purification are :1.Extraction2.Precipitation and differential Solubilization3.Ultracentrifugation4.Chromatographic MethodsFURTHER CLASSIFICATIONCHROMATOGRAPHIC METHOD :1. Size Exclusion Chromatography2. Ion Exchange Chromatography3. Affinity Chromatography4.Metal Bindin … Purchase document to see full attachment

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